(1. 中南大學(xué) 資源加工與生物工程學(xué)院，教育部生物冶金重點(diǎn)實(shí)驗(yàn)室，長(zhǎng)沙 410083；
2. 東北大學(xué) 冶金學(xué)院，沈陽(yáng) 110819)
摘 要: 為了獲得耐砷能力較高的中度嗜熱嗜酸浸礦菌Leptospirillum ferriphilum，采用連續(xù)轉(zhuǎn)接馴化法進(jìn)行耐砷馴化，并對(duì)菌株的馴化機(jī)制進(jìn)行研究。結(jié)果表明：馴化后Leptospririllum ferriphilum菌的As(Ⅲ)和As(Ⅴ)耐受濃度分別提高到7.0 g/L和11.0g/L；As(Ⅴ)馴化菌株對(duì)砷黃鐵礦的浸出作用明顯比As(Ⅲ)馴化菌株強(qiáng)；砷馴化菌細(xì)胞官能團(tuán)發(fā)生明顯變化，多糖和蛋白質(zhì)含量明顯增多，細(xì)胞表面負(fù)電荷明顯降低，以適應(yīng)高砷的環(huán)境。同步輻射原位C-K邊XANES譜圖表明，馴化菌糖類(lèi)和蛋白質(zhì)的分布位置發(fā)生了變化；DFT理論計(jì)算表明，馴化菌更容易吸附到礦物表面上。
關(guān)鍵字: 砷；砷黃鐵礦；黃鐵礦；馴化
（1. Key Laboratory of Biometallurgy of Ministry of Education of China, School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China;
2. School of Metallurgy, Northeastern Univwrsity, Shenyang 110819, China）
Abstract:In order to obtain high arsenic-tolerant ability of Leptospirillum ferriphilum, continuous transfer domestication was adopted, and the acclimation mechanism was studied. The results show that, after domestication, the tolerant abilities to As(Ⅲ) and As(Ⅴ) of L. ferriphilum increase to 7.0 g/L and 11.0 g/L, respectively. The leaching effect of the bacteria acclimated by As(Ⅴ) is significantly higher than that by As(Ⅲ) in the leaching of arsenopyrite. After being acclimated, the contents of polysaccharide and protein of cells significantly increase, while the negative charge on the surface of cells significantly decreases, so as to adapt to the high arsenic environment. The in-situ C-K-edge XANES results show a change in the distribution of polysaccharide and protein. The DFT calculation shows that the adapted bacterial cells are easier to be adsorbed on the mineral surface.
Key words: arsenic; arsenopyrite; pyrite; domestication
