(1. 南華大學(xué) 核資源工程學(xué)院，衡陽 421001；
2. 南華大學(xué) 鈾礦冶生物技術(shù)國防重點學(xué)科實驗室，衡陽 421001；
3. 中南大學(xué) 資源加工與生物工程學(xué)院，長沙 410083)
摘 要: 為了探明混合浸礦微生物的耐氟特性及其群落動態(tài)，構(gòu)建5株浸礦細菌(Acidithiobacillus ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01, Acidithiobacills caldus S1)的共培養(yǎng)體系。結(jié)果表明：氟脅迫在一定程度上抑制了該共培養(yǎng)體系的生長繁殖，對于高濃度的氟脅迫，共培養(yǎng)體系也保持較為平穩(wěn)的生長延滯狀態(tài)；氟脅迫對共培養(yǎng)體系硫氧化活性的影響并不明顯。采用實時熒光定量PCR技術(shù)對其培養(yǎng)體系的群落動態(tài)進行分析。結(jié)果表明，不論是氟脅迫下還是無氟脅迫下，L. ferriphilum YSK和A. caldus S1，都是群落中的優(yōu)勢種群；而A. ferrooxidans ATCC 23270、S. thermosulfidooxidans ST和A. thiooxidans A01為劣勢種群。共培養(yǎng)體系中受氟脅迫影響最大的是S. thermosulfidooxidans ST，菌體密度急劇下降；其次是A. caldus S1、A. thiooxidans A01和A. ferrooxidans ATCC 23270，氟脅迫在一定程度上也抑制了對它們的生長；而L. ferriphilum YSK在氟脅迫下的生長非常穩(wěn)定。
關(guān)鍵字: 浸礦微生物；共培養(yǎng)體系；氟耐受性；群落動態(tài)
（1. School of Nuclear Resources Engineering, University of South China, Hengyang 421001, China;
2. Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,
University of South China, Hengyang 421001, China;
3. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:In order to explore the fluoride tolerance and the community dynamics of mixed bioleaching microorganisms, a co-culture including five typical bioleaching strains (Acidithiobacillus ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01, Acidithiobacills caldus S1) was constructed. The results show that the growth of the co-culture is inhibited by fluoride stress, and the co-culture keeps the stable cell density at high fluoride stress. The sulfur activity of the co-culture is not affected by fluoride stress. Microbial community dynamics of the co-culture was analyzed by real-time fluorescent quantitative PCR technology. The results show that L. ferriphilum YSK and A. caldus S1 are always the dominant species at fluoride stress or non-stress, while the minor is A. ferrooxidans ATCC 23270, S. thermosulfidooxidans ST and A. thiooxidans A01. Besides, S. thermosulfidooxidans ST is inhibited most obviously in the co-culture at fluoride stress, secondly A. caldus S1, A. thiooxidans A01 and A. ferrooxidans ATCC 23270, while the L. ferriphilum YSK still maintains a very stable growth.
Key words: bioleaching microorganisms; co-culture; fluoride tolerance; community dynamics
