(1. 北京科技大學(xué) 土木與環(huán)境工程學(xué)院，北京 100083；
2. 中國科學(xué)院過程工程研究所 生化工程國家重點(diǎn)實(shí)驗(yàn)室，北京 100190)
摘 要: 嗜酸氧化亞鐵硫桿菌(Acidithiobacillus ferrooxidans: A. ferrooxidans菌)是目前研究得最多的浸礦細(xì)菌，其能量代謝途徑復(fù)雜多樣。在好氧和厭氧氣氛下，分別對(duì)Fe3+浸出黃鐵礦及A. ferrooxidans菌對(duì)Fe3+氧化浸出黃鐵礦的影響進(jìn)行了研究，并且利用A. ferrooxidans菌構(gòu)建微生物燃料電池，研究在不同氣氛下A. ferrooxidans菌對(duì)電子傳遞過程的影響。結(jié)果表明：在好氧和厭氧氣氛下，加菌時(shí)的黃鐵礦浸出率比無菌時(shí)的分別提高了40.03%和27.76%。在好氧和厭氧氣氛下，A. ferrooxidans菌均可以提高電子傳遞速率，進(jìn)而加快氧化還原反應(yīng)的進(jìn)行，說明A. ferrooxidans菌在厭氧環(huán)境下，能以Fe3+為電子受體、含還原性硫的黃鐵礦為電子供體來進(jìn)行呼吸作用，獲得生命活動(dòng)所需的能量。在實(shí)驗(yàn)結(jié)果和前人工作的基礎(chǔ)上提出在厭氧情況下，A. ferrooxidans菌進(jìn)行呼吸作用的一條可能的路線圖。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌；鐵氧化系統(tǒng)；硫氧化系統(tǒng)；電子傳遞
（1. School of Civil and Environmental Engineering, University of Science and
Technology Beijing, Beijing 100083, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Proce）
Abstract:Acidithiobacillus ferrooxidans (A. ferrooxidans) is the most widely studied bacteria, which is used in leaching ores work. The pyrite leached by ferric iron and the effect of A. ferrooxidans on pyrite leaching were studied under the aerobic and anaerobic atmosphere, respectively. Bio-cathode microbial fuel cells were constructed in accordance with the characteristics of A. ferrooxidans, and then used to study the effect of A. ferrooxidans on the electron transfer process under the aerobic and anaerobic atmosphere. The results show that, under the aerobic and anaerobic atmosphere, the leaching rates of pyrite are 40.03% and 27.76% more than those of chemical leaching, respectively. Under the aerobic and anaerobic atmosphere, the presence of A. ferrooxidans both can improve the electron transfer rate, and thus speed up the conduct of the redox reaction. This demonstrates that, in the anaerobic environment, A. ferrooxidans can be respiration using Fe3+ as electron acceptor and pyrite containing reduced sulfur as electron donor. On the basis of experimental results and previous work, a possible electron transport chain of A. ferrooxidans under anaerobic condition was proposed.
Key words: Acidithiobacillus ferrooxidans; iron oxide system; sulfur oxide system; electron transport
