(1. 中南大學 冶金科學與工程學院，長沙 410017；
2. 中機國際工程設計研究院有限責任公司，長沙 410007)
摘 要: 以pannonibacter phragmitetus T1菌作為生物吸附劑，研究細菌代謝活性、培養(yǎng)時間、溶液pH值、菌體用量、溫度、吸附時間、初始金屬濃度等因素對其吸附重金屬Pb2+的影響，并進一步研究吸附機理。結果表明，培養(yǎng)12 h的滅活T1菌體在Pb2+濃度為150 mg/L、pH值為6、T1菌添加量為0.5 g/L、溫度為30 ℃的條件下，對Pb2+的最大吸附量為68.35 mg/g；滅活T1菌對重金屬Pb2+的吸附過程較快，90 min即達到吸附平衡，Pb2+的吸附符合Pseudo-second order模型和Langmuir等溫吸附模型。結合紅外光譜分析，滅活pannonibacter phragmitetus T1菌對Pb2+是表面吸附，羥基和酰胺基是與Pb2+絡合反應的主要基團。
關鍵字: pannonibacter phragmitetus T1菌；鉛離子；吸附條件；吸附機理
（1. School of Metallurgical Science and Engineering, Central South University, Changsha 410017, China;
2. China Machinery International Engineering Design & Research Institute Co., Ltd., Changsha 410007, China）
Abstract:The pannonibacter phragmitetus T1 was used as bio-sorbent, the effects of the factors such as the metabolic activity of bacteria, culture time, pH value, biomass concentration, temperature, adsorption time and initial concentration of metal ions on the biosorption of Pb2+ were determined, and the adsorption mechanism was further discussed. The results show that the maximum adsorption capacity of T1 for Pb2+ reaches 68.35 mg/g under the optimal conditions as the Pb2+ concentration of 150 mg/L, pH value of 6, the nonliving bacteria cultured after 12 h with the biomass of 0.5 g/L, and temperature of 30 ℃. The kinetic study of the adsorption shows that the adsorption process of Pb2+ is rapid, and finishes in 90 min. The adsorption process is well accorded with the Pseudo-second order model and Langmuir model. The analysis of the FTIR spectrum indicates that the hydroxyl and amide are the main reactive groups on the surface adsorption.
Key words: pannonibacter phragmitetus T1; Pb2+; biosorption condition; biosorption mechanism
