(1. 中南大學 粉末冶金國家重點實驗室，長沙 410083；
2. 中南大學 湘雅三醫(yī)院，長沙 410013；
3. 中南大學 醫(yī)用材料與器械研究中心，長沙 410013)
摘 要: 采用水熱合成法制備經(jīng)精氨酸表面修飾的羥基磷灰石納米顆粒(Arg-nHAP)。利用透射電鏡(TEM)、Zeta電位分析儀、傅立葉紅外光譜儀和X射線衍射(XRD)對Arg-nHAP的形貌、結(jié)構(gòu)、晶粒粒徑和Zeta電位進行表征，并將其與質(zhì)粒DNA(pEGFP-N1)進行凝膠電泳實驗，研究Arg-nHAP對基因的結(jié)合和保護性能。結(jié)果表明：制備的Arg-nHAP粒徑較均勻，約為50 nm~80 nm，分散性良好；在pH=7.4時，納米顆粒的表面凈電荷均值為30.89 mV，可與帶負電荷的質(zhì)粒DNA通過靜電結(jié)合快速形成納米顆粒−質(zhì)粒DNA復合物，對結(jié)合的DNA起到明顯的保護作用；經(jīng)精氨酸表面修飾的Arg-nHAP可成為一種有效的基因結(jié)合載體。
關(guān)鍵字: 羥基磷灰石；精氨酸；水熱合成；基因載體
hydroxyapatite nanoparticles
（1. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
2. The Third Xiangya Hospital, Central South University, Changsha 410013, China;
3. Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China）
Abstract:An arginine modifying nano-hydroxyapatite (nHAP) was synthesized by hydrothermal synthesis. The morphology, structure, crystallite size and Zeta potential of the Arg-nHAP were characterized by X-ray diffractometry(XRD), transmission electron microscopy(TEM), Fourier transform infrared (FTIR) and Zeta potential analyzer. The binding and protecting properties of Arg-nHAP to DNA were tested by electrophoresis experiment. The results show that the Arg-nHAP is very fine and well distributes, the mean diameter of the nanoparticles is 50−80 nm and its Zeta potential is about 30.89 mV at pH of 7.4. Arg-nHAP/DNA nanocomplexes can be formed rapidly by electrostatic interaction of nanoparticles with DNA having negative electrons. The nHAP modified with arginine can effectively bind and protect DNA and may be used as a potential gene carrier.
Key words: hydroxyapatite; arginine; hydrothermal synthesis; gene carrier
