(中南大學 資源加工與生物工程學院 生物冶金教育部重點實驗室，長沙 410083)
摘 要: 以嗜酸氧化亞鐵硫桿菌(Acidithiobacillus ferrooxidans, ATCC23270)為對象，研究NO3−、SO42−、Cl−和PO43−對Acidithiobacillus ferrooxidans生長和硫氧化活性的影響。結(jié)果表明：不同陰離子對Acidithiobacillus ferrooxidans硫氧化表觀活性的影響效應不同，其影響由大到小的順序依次為Cl−，NO3−，SO42−和PO43−；在高濃度PO43−的脅迫(300 mmol/L)下，細菌所表達蛋白質(zhì)的總數(shù)量減少，但同時表達一些特異性蛋白質(zhì)，這說明細菌可通過抑制一些在正常條件下表達的蛋白質(zhì)，同時表達一些新的蛋白質(zhì)來應對無機離子的脅迫效應。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌；陰離子；脅迫效應
Acidithiobacillus ferrooxidans
（Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering,
Central South University, Changsha 410083, China）
Abstract:The effects of NO3−, SO42−, Cl− and PO43− on the growth of Acidithiobacillus ferrooxidans and sulfur oxidation activity were investigated. The results show that the anions have different effects on the sulfur oxidation activity, and the inhibition effect sequence from great to little is Cl−, NO3−, SO42−, PO43−. The total proteins of Acidithiobacillus ferrooxidans grown under PO43− threatening condition (300 mmol/L) are down-regulated, but specific proteins are observed, when compared with normal condition. These specific proteins may take part in the response to the stress effect of anions.
Key words: Acidithiobacillus ferrooxidans; anion; stress effect
