(中國科學(xué)院 化工冶金研究所， 北京 100080)
摘 要: 研究了在常用的細(xì)菌浸出條件下( t =30 ℃, pH 2.0)， Mg2+對氧化亞鐵硫桿菌(T.f)生長活性的影響。 結(jié)果表明， 該實驗所用菌株具有一定的耐Mg2+能力；當(dāng)培養(yǎng)基中 ρ (Mg2+ )≤10.5 g/L時， Mg2+對細(xì)菌的生長活性沒有不利的影響； ρ (Mg2+ )15.5 g/L時對細(xì)菌生長活性的影響較明顯； ρ (Mg2+)20.5 g/L時細(xì)菌的生長完全受到抑制。 經(jīng)馴化后的該菌株耐Mg2+性明顯提高，在含ρ (Mg2+ )15.5 g/L的培養(yǎng)基中細(xì)菌的生長活性良好。
關(guān)鍵字: 氧化亞鐵硫桿菌； 生長活性； Mg2+ 的影響
（Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, P.R.China）
Abstract: The effect of Mg2+ on the growth and activity of Thiobacillus ferrooxidans (T. f) was studied under the common bioleaching condition of 30℃ and pH 2.0. Experimental results show that the T.f used in this experiment was tolerant of Mg2+ to some extent. No bad effect on the growth and activity of T.f by Mg2+ was brought when cultured in Leathen medium containing ρ (Mg2+ )≤10.5 g/L. The growth and activity of T.f are obviously influenced when ρ ( Mg2+ )15.5 g/L. But the growth and activity of T.f cease when the concentration of Mg2+ is around 20.5 g/L. The T.f can tolerate the concentration of Mg2+ 15.5 g/L in the culturemedium after a period of adaptation by repeated subculturing.
Key words: Thiobacillus ferrooxidans ; growth and activity; tolerance of Mg2+
