(1. 中南大學(xué) 粉末冶金國(guó)家重點(diǎn)實(shí)驗(yàn)室，長(zhǎng)沙 410083;
2. Bioengineering Unit，Strathclyde University In Glasgow，
Glasgow G4 ONW, UK)
摘 要: 采用體外細(xì)胞培養(yǎng)模式，通過測(cè)定細(xì)胞堿式磷酸酶(ALP)活性及細(xì)胞四甲基偶氮砜(MTT)還原能力， 對(duì)構(gòu)成金屬和合金生物材料的金屬離子(Al， Ni， Cr， V， Ag)的毒性進(jìn)行了定量評(píng)估，探討了金屬離子或原子對(duì)人體組織的毒害和腐蝕作用。結(jié)果表明微量六價(jià)鉻離子表現(xiàn)出強(qiáng)烈的細(xì)胞毒性。當(dāng)溶液離子度高于100μmol/L時(shí)，釩和鎳亦表現(xiàn)強(qiáng)烈毒性。銀離子和三價(jià)鉻離子濃度高于500μmol/L后表現(xiàn)毒性。構(gòu)成老年癡呆病成因的金屬鋁離子的毒性具有雙向性。
關(guān)鍵字: 生物材料； 金屬毒性； 細(xì)胞分子生物學(xué)
（1. State Key Laboratory for Powder Metallurgy，
Central South University，Changsha 410083, P.R.China;
2 Bioengineering Unit， Strathclyde University，
Glasgow G4 ONW， UK）
Abstract:Cytoxicity analysis is the critical testing of evaluating biomaterials in the ASTM，ISO standards. The approach has invested in vitro the toxicity of metal ions (Al， Ni， Cr(Ⅲ)， Cr(Ⅵ)， V， and Ag) that construct the metallic biomaterials. By measurements of cell alkline activity (ALP) and reduction ability of cell MTT， the toxicity of prevalence metallicbiomaterials has been studied， and furthermore， the poison and erosion of metal ions and atoms on human tissue are discussed. The results display that trace Cr(Ⅵ) shows serious cytotoxicity, and V as well as Ni is cytotoxicity if the ion concentration in culture medium is over 100μmol/L. A strange phenomenon is that Ag is also cytotoxicity if the ion concentration is higher than 500μmol/L. Al ions is biphasic in cytotoxicity. At low ion concentration (<10μmol/L)， Al ions can stimulate cell proliferation， whereas concentration is over 1000μmol/L， cytotoxicity increases.
Key words: biomaterials; metal toxicity; cell biological molecular
