(蘭州大學(xué) 生命科學(xué)學(xué)院， 蘭州 730000)
摘 要: 以氧化亞鐵硫桿菌野生菌（wide T.f）和馴化菌（adapted T.f）為供試菌株， 通過比較野生菌與馴化菌對(duì)Cu2+的吸收與運(yùn)輸特性，研究了氧化亞鐵硫桿菌馴化菌株的抗銅機(jī)理。 結(jié)果表明： Cu2+對(duì)野生菌Fe2+的氧化活性影響較大， 當(dāng)Cu2+濃度高于10 g/L時(shí)，細(xì)菌Fe2+氧化活性逐漸降低直至完全抑制； 而經(jīng)馴化培養(yǎng)的T.f菌， 對(duì)重金屬銅則具有較高的抗性， 其Fe2+氧化活性也較高， Fe2+氧化速率加快； 在不同Cu2+濃度下，馴化菌細(xì)胞中的Cu2+含量總低于野生菌的， 經(jīng)馴化培養(yǎng)的氧化亞鐵硫桿菌與野生菌相比， 具有更高的促進(jìn)體內(nèi)
Cu2+排出的能力， 該排出系統(tǒng)與ATPase的活性有關(guān)。
關(guān)鍵字: 氧化亞鐵硫桿菌； 銅離子； 運(yùn)輸特性； 氧化活性
in adapted Thiobacillus ferrooxidans
（ School of Life Science, Lanzhou University, Lanzhou 730000, China）
Abstract: Copper-resistance mechanisms of the adapted Thiobacillus ferrooxidans were studied in this thesis by comparing the uptake and transport characterization with Cu2+ in the wide T.f and the adapted T.f. The results show that wide T.f is largely affected by Cu2+ when the content of Cu2+ is bigger than
10 g/L, the active oxidation of Fe2+ reduces gradually till completely restraining. The adapted T.f is quite resistant to the heavy metal copper, exhibiting in the active oxidation of Fe2+ and its speeding oxidation rate. Further, the accumulation of Cu2+ in the cell of the adapted T.f is always lower than the wide T.f. Compared with the wide T.f, the adapted T.f possesses higher power in exporting Cu2+ and the efflux system is related to the activity of ATPase.
Key words: Thiobacillus ferrooxidans; Cu2+ ion; transportation characterization; oxidation activity
