中國有色金屬學(xué)報(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 19 No. 3 June 2009 |
QIU Guan-zhou(邱冠周)1, ZHOU Ji-zhong(周集中)1, 2, LIU Xue-duan(劉學(xué)端)1
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma,
Norman 73019, USA）
Abstract:Biofilm systems are effective for biosorption of metal ions. Shewanella oneidensis MR-1, a Gram-negative facultative anaerobe, is a natural pellicle-like biofilm former. The mechanisms of pellicle formation by S. oneidensis MR-1 have not yet been understood. 17 S. oneidensis MR-1 deletion mutants, including 12 c-type cytochromes were generated and tested if they were involved in pellicle formation. The results show that ∆SO4666, ∆SO1777, ∆SO1782, ∆SO2361 and ∆SO2363 have varying deficiency in pellicle formation. The deletion mutant ∆SO4666 cannot form a pellicle under non-shake conditions, suggesting that it may play an important role in pellicle formation by S. oneidensis MR-1. Overall, these data suggest a very complex picture of aerobic respiration by S. oneidensis MR-1.
Key words: cytochrome; mutant; electron transport; pellicle; biofilms
